Diagnostic value of next-generation sequencing to detect periprosthetic joint infection

Abstract Background We herein compared the diagnostic value of next-generation sequencing (NGS), bacterial culture, and serological biomarkers to detect periprosthetic joint infection (PJI) after replacement. Methods According criteria Musculoskeletal Infection Society, 35 patients who underwent revision surgery were divided into (15 cases) non-infection (20 groups, routinely examined preoperatively for erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), D-dimer levels. All arthrocentesis preoperatively. Synovial fluid was used white blood cell count, classification, NGS. Furthermore, we calculated area under curve (AUC) receiver operating characteristic (ROC) ESR, CRP, PCT, IL-6, D-dimer. Data assessed by comparing accuracy, sensitivity, specificity. Results Fourteen showed positive results NGS seven culture in group; further, 18 negative group. The AUC D-dimer, PCT 0.667, 0.572, 0.827, 0.767, 0.808, respectively. accuracy NGS, 0.91, 0.74, 0.77, 0.83, When with differences overall test those sensitivity statistically significant, specificity also significant. In comparison insignificant. Conclusions Our indicated that had higher than method commonly diagnosing PJI.